Figure 7. Examples of genes whose expression was preferentially stimulated by E2 or BEs in cells containing ERα+ERβ compared with cells containing ERα only or ERβ only.

Cells were treated with control vehicle (0.1% ethanol), 10 nM E2, or 1 µM genistein, S-equol, or liquiritigenin for 24 h prior to harvest and real-time PCR analysis. mRNA fold change is shown for specific genes in the three cell types. Real-time PCR data shown are mean ± SD for triplicate analyses.