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Exposure of VPS35 P316S and D620N mutant flies to rotenone accelerates DA neuronal loss. (A) Bar graph shows the quantification of the number of DA neurons in different clusters of various fly species 15 days after rotenone treatment (n = 3, cohort of 10). (B) Representative confocal images showing TH-positive DA neurons in the PPL1 cluster of rotenone treated fly species in VPS35 wild type, P316S and D620N flies.
