Figure 3.

Interaction analyses among OpsDHN1 (SK₃) and truncated versions (SK₂ and S) using the split-ubiquitin yeast two-hybrid assay. (A) Schematic representations of OpsDHN1 derived versions used in the two-hybrid split ubiquitin system. OpsDHN1 SK₃ full version; OpsDHN1 SK₂ C-terminal truncated version (residues 1–199); OpsDHN1 S short version (residues 1–97). The capital letters indicates characteristic motifs of OpsDHN1 protein: S (S-segment), K (K-segment), and H (histidine-rich motif). (B) Yeast cells carrying the control system interaction LargeT-Cub/Δp53-NubG. Interaction analysis among baits and preys OpsDHN1 derived versions (SK3, SK2, and S). Yeast strains was plated to an OD600 of 0.8, and at serial 10-fold dilutions on semi-selective (SD-LW) and on selective (SD-LWHA) media supplemented with 3-AT (45 and 55 mM). Quantitative β-Galactosidase activity was assayed by hydrolysis of the o-nitrophenyl-b-galactoside (ONPG), as expressed in nmol ONP/min per mg of protein. Data represent the mean ± SD, (n = 3).