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. 2014 Sep 25;13:150. doi: 10.1186/1476-511X-13-150

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© Iino et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of S1P and LPA receptors in Jurkat cells. (A) The expression of S1Ps and LPAs in Jurkat cells was examined by RT-PCR. The amplified products for the Jurkat mRNAs for S1P and LPA receptors were resolved in a 2.5% agarose gel. MW indicates the molecular size markers. mRNA transcripts for S1P₁, S1P₂, S1P_3, and S1P₄, were found, but that of S1P₅ could be hardly detected. Also, LPA₁, LPA₂, LPA_3, and LPA₄ were expressed. (B) The surface expression of S1P₁ in Jurkat cells was examined by flow-cytometry. Horizontal axis indicates the strength of S1P₁ expression as assessed by the indirect FITC fluorescence intensity. The bold line histogram represents staining with anti-S1P₁ antibody, while the narrow line represents the negative control. S1P₁ was found expressed on Jurkat cells.