Figure 3.

Aptamer based reusable ECPA for thrombin quantitation. (A) SWV scans for thrombin quantitation in the pM to nM range. Seven different sensors responded proportionally to thrombin, suggesting high method precision. (B) Thrombin calibration curves with seven different sensors (dotted lines), with the average trace (black line) shown; error bars represent standard deviations. The thrombin LOD was found to be 10 pM. (C) Two independent sensors were cycled through the reusable ECPA methodology during measurement of differing thrombin concentrations (red and black points). The gentle enzymatic surface regeneration permitted up to 19 uses without significant loss of signal. (D) High target specificity was confirmed through various challenges to the sensor, where only thrombin generated measurable responses.