Table 1.
Effect on invasion of S. dysenteriae. S. flexneri and S. sonnei of whey, casein, PIS (sIgA), P2S (Lf, SC and albumin, SA), P3S (α-lactoalbumin), P2H (fSC and SA) and P3H (Lf).
S. dysenteriae wild type | S. flexneri wild type | S. sonnei wild type | ||||||||||
Bacteria | n°/Hela cell | % inhibition | p | bacteria | n°/Hela cell | % inhibition | p | bacteria | n°/Hela cell | % inhibition | p | |
Fraction | control | Tested | control | tested | control | tested | ||||||
Whey | 5.2 ± 0,4 | 2.2 ± 0,2 | 58 | 0.001 | 0.68 ± 0.2 | 0.28 ± 0.2 | 74 | 0.007 | 0.49 ± 0.24 | 0.18 ± 0.15 | 62 | 0.008 |
casein | 6.6 ± 1,0 | 6.5 ± 1,1 | - | ns | 0.89 ± 0.1 | 0.83 ± 0.2 | - | ns | 0.53 ± 0.10 | 0.52 ± 0.12 | - | ns |
slgA | 6.1 ± 0,1 | 2.7 ± 0,2 | 44 | 0.006 | 1. 60 ± 0.08 | 0.56 ± 0.08 | 58 | 0.009 | 1.98 ± 0.60 | 1.15 ± 0.09 | 42 | 0.005 |
Lf+SC+AS | 6.6 ± 0,4 | 2.7 ± 0,2 | 59 | 0.008 | 0.79 ± 0.04 | 0.51 ± 0.04 | 36 | 0.008 | 0.70 ± 0.05 | 0.16 ± 0.05 | 77 | 0.007 |
α-lactoalbumin | 7.2 ± 0,4 | 7.1 ± 0,2 | - | ns | 0.81 ± 0.05 | 0.79 ± 0.02 | - | ns | 0.92 ± 0.60 | 0.85 ± 0.45 | - | ns |
fSC | 5.8 ± O,5 | 3.4 ± 0,2 | 42 | 0.009 | 1,3 ± 0.02 | 0.75 ± 0.04 | 42 | 0.003 | 2.00 ± 0.05 | 0.84 ± 0.04 | 58 | 0.01 |
Lf | 6.2 ± 0,8 | 3.2 ± 0,1 | 48 | 0.002 | 0.8 ± 0.09 | 0.42 ± 0.05 | 48 | 0.004 | 1.80 ± 0.02 | 0.65 ± 0.10 | 64 | 0.03 |
ns = not significative. p = statistical analyses (t-Student independent)