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Sap47 gene suppression by the two RNAi techniques used is similar. The Western blot shows SAP47 protein levels in three independent cDNA-cDNA RNAi lines (1: line VII.5 (2 transgene insertions), 2: line VII.10 (2 transgene insertions), 3: line X.2) and in one genomic-cDNA RNAi line (4: line 2.4) WT: wild-type control; one head/lane. All RNAi constructs are under actin-Gal4 control. CSP: loading control.
