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. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Cell. 2008 Apr 18;133(2):314–327. doi: 10.1016/j.cell.2008.02.030

Figure 1. WT Upf1 Preferentially Interacts with SMG-1 and eRF3, whereas Phospho-Upf1 Preferentially Interacts with mRNA Decay Factors.

Figure 1

(A) HeLa cells (3–4 × 107) were transiently transfected with pCMV-myc-UPF1R(WT) or pCMV-myc-UPF1R(G495R/G497E) (10 µg). Two days later, total-cell lysate was immunoprecipitated using anti(α)-myc or mouse (m)IgG. Western blotting (WB) was performed using the specified antibodies. The four leftmost lanes analyzed decreasing amounts of protein before (−) IP.

(B) Essentially as in (A), except cells were transiently transfected with pCMV-myc-UPF1R(WT) and treated with the specified amount of OA for 3 hr prior to harvesting. All results are representative of three independently performed experiments.