Skip to main content
. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: J Blood Disord Transfus. 2013 Sep 20;4(5):158. doi: 10.4172/2155-9864.1000158

Table 1.

Formulations of the Gibco recovery medium and the custom medium used for cryopreservation of PBMC for 7 days at −65°C and −80°C.

Gibco Medium Custom Medium

  1. High-glucose Dulbecco’s Modified Eagle Medium (DMEM) −80%

    • Contains high glucose, non-essential amino acids, sodium pyruvate, phenol red

  2. Bovine Serum and FBS −10%

  3. DMSO-10%

  1. RPMI-1640 (12–702F)-27.5%

    • Contains sodium bicarbonate (NaHCO3) 26.7 ml/L of 7.5% NaHCO3 and 10.3 ml of 200 mM L-glutamine

  2. FBS-62.5%

  3. DMSO-10%