Figure 7. Ang II potentiates prorenin-induced TNFα production in microglia.

Mouse N-9 cells were pretreated with control media (DMEM) or Ang II (100 nmol/L) for 12 h, followed by treatment with DMEM or prorenin (20 nmol/L) for 6 hr. Next, TNFα positive cells were analyzed by flow cytometry. Data are means ± SEM from n = 6 experiments. * P<0.05 vs. control; † P<0.05 vs. prorenin without Ang II pretreatment.