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. 2014 Oct 10;9(10):e108593. doi: 10.1371/journal.pone.0108593

Figure 2. Inhibition of SRF by CCG-1423 ameliorates PD-induced PM fibrosis in vivo.

Figure 2

(a) Rats received a daily instillation of PD fluid or saline for 6 weeks. After that period, samples were prepared and analyzed as described in the Concise Methods section. (a1) As measured by HE staining, saline exposure resulted in little change in PM. (a2) Exposure to 4.25% PD fluid resulted in an increase in the thickness of the PM. (a3). CCG-1423 treatment significantly reduced HG-PD effects. The histogram showed the thickness for HE staining in the submesothelial compact zone (mean±SE, n = 8). Magnification is 200×. (b) Staining of the EMT markers E-cadherin, α-SMA and SRF in peritoneal samples by immunohistochemistry reveals that PD fluid exposure induces the EMT process, while CCG-1423 treatment significantly ameliorated EMT-derived fibrosis. Magnification is 200×.