Figure 2. Cdh5-Cre drives efficient deletion of podocalyxin in the lung.

(A) Podocalyxin expression is completely abrogated in the lung of Podxl ^ΔEC mice. (B, C) Within the aorta and vessels of the small intestine (arrows), podocalyxin is deleted in all but a few isolated cells in Podxl ^ΔEC mice. (D) Within the kidney, podocalyxin (brown staining) is efficiently deleted in the glomerular endothelial cells (arrows, 40x mag. panels) and larger vessels (arrowheads, 10x mag. panels). Positive staining in the glomerulus of Podxl ^ΔEC kidney are likely podocyte epithelial cells. Adjacent H&E sections included demonstrating normal morphology with loss of podocalyxin expression in these tissues.