Skip to main content
. 2014 Oct 10;9(10):e108881. doi: 10.1371/journal.pone.0108881

Figure 4. Deletion of vascular podocalyxin contributes to structural and functional changes in the lung.

Figure 4

(A) H&E stained sections of lungs inflated to 25 cm H2O with open thoracic cavity from PodxlF/F and Podxl ΔEC mice obtained at 4 weeks and 10 weeks post-natal. Loss of podocalyxin results in increased mean linear intercept (MLI) at 10 weeks of age. The MLI values were determined by computer-assisted image analysis (n = 6 mice per genotype). (B) Mean lung volumes of PodxlF/F and Podxl ΔEC mice at 2, 4 and 10 weeks (n = 4–6 mice per genotype). Representative images of inflated lungs at 4 and 10 weeks are shown below the graphs. (C) H&E stained sections of lungs inflated with constant volume with closed chest wall from adult PodxlF/F and Podxl ΔEC mice (n = 6 mice per genotype). (D) Resistance measurements from primewave-8 (Rn) and snapshot (R) perturbation. *Significantly different with P<0.05 by Student's t test; **Significantly different with P<0.01 by Student's t test at each time point. The error bars represent the SEM.