Figure 4. Progress curves for the activation of diphenolase of mushroom tyrosinase by α-arbutin.

. The reaction media (3.0 mL) contained 0.5 mM L-Dopa in 50 mM phosphate buffer (pH 6.8), the indicated concentration of α-arbutin, and mushroom tyrosinase (6.67 µg/mL). The concentrations of α-arbutin for curves 1∼3 were 0, 5, 10 mmol·L⁻¹.