|
Strains and plasmids
|
| Yb2 |
Riemerella anatipestifer serotype 2 strain |
This study |
|
Escherichia coli S17–1 |
lpir hsdR pro thi; chromosomal integrated RP4–2 Tc::Mu Km::Tn7 |
17 |
| RA2640 |
Tn4351 insertion mutant of Riemerella. anatipestifer Yb2, AS87_04050::Tn |
This study |
| cRA2640 |
Mutant RA2640 carrying plasmid pCP29- AS87_04050 |
This study |
| pCP29 |
ColE1 ori; (pCP1 ori); Apr(Emr); E. coli-F. johnsoniae shuttle plasmid |
17 |
| pCP- AS87_04050 |
pCP29 containing ompA promoter and AS87_04050 ORF, cfxAr (Apr) |
This study |
|
Primers
|
|
|
| RA 16S rRNA-F |
5′-GGAGGCAGCAGTGAGGAATA-3′
|
This study |
| RA 16S rRNA-R |
5′-ATCGTTTACGGCGTGGACTA-3′
|
This study |
| Erm-F |
5′-GCCCGAAATGTTCAAGTTGT-3′
|
This study |
| Erm-R |
5′-CTTGACAACCACCCGACTTT-3′
|
This study |
| Tn4351-F |
5′ TGGCACCTTTGTGGTTCTTAC 3′ |
This study |
| Tn4351-R |
5′ GAGAGACAATGTCCCCCTTTC 3′ |
This study |
| AS87_04040 F |
5′-TCATGCAGTTTTGGAAGCAG-3′
|
This study |
| AS87_04040 R |
5′-CGACGACCAGCCAAAATAAT-3′
|
This study |
| AS87_04050 F |
5′-TTGGTAACGATTGGGAATCTC-3′
|
This study |
| AS87_04050 R |
5′-TTACATCAGGCCGCTTTAGG-3′
|
This study |
| AS87_04055 F |
5′-TATCTGGCTTAGGCGGAATG-3′
|
This study |
| AS87_04055 R |
5′-GGTGAGGTTTCCCCGTAAAG-3′
|
This study |
| RA ldh F |
5′-AACTTCCGCTTGGTATGCAC-3′
|
This study |
| RA ldh R |
5′-TAGCCGCAGTAGCGAATTTT-3′
|
This study |
| ompA promoter P1 |
5′-CAGGTACCATAGCTAAAATTTTGGCAGTAAC-3′ (Kpn I site underlined) |
17 |
| ompA promoter P2 |
5′-CGACTCGAGCATTCCAATTCTCTTATTATC-3′ (Xho I site underlined) |
17 |
| AS87_04050 -comp-F |
5′-CCGCTCGAGATGAATAACAGGAAAATATTAATTACTGGAGGGGCAGG-3′ (Xho I site underlined) |
This study |
| AS87_04050 -comp-R |
5′-CATGCATGCTTATTTCAACCTCTTCCAATACCAATCCAC-3′ (Sph I site underlined) |
This study |
