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. 2014 Oct 10;9(10):e109844. doi: 10.1371/journal.pone.0109844

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© 2014 Rico et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Colony morphology of S. coelicolor M145 and S. coelicolor ΔabrA1/A2 strains transformed with different multicopy plasmids: pIJ702 (control), pTXabrA2 (expressing the AbrA2 RR under the control of xysAp), and its derivatives pTXabrA2-DA (D₅₅A) and pTXabrA2-DADE (D₁₀A, D₅₅E). The photographs correspond to four-day cultures on R2(YE) medium. Bar: 1 mm. B) Phenotypes of AbrA2 overexpression in S. coelicolor M145 compared to the mutant ΔabrA1/A2 phenotype. The phosphoablative version (pTXabrA2-DA) was included in the study. Upper part: production of ACT in NMMP medium plates (72 h); middle part: CDA bioassay against B. subtilis at 48 h; lower part, RED production at 48 h.