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. 2014 Oct 10;9(10):e109853. doi: 10.1371/journal.pone.0109853

Figure 4. EDS1 protein but not mRNA levels are constitutively higher in the cir1 mutant.

Figure 4

(A) Total protein from 4-week-old plants grown at 22°C was separated by SDS–PAGE, transferred to nitrocellulose membrane and probed with an EDS1 antibody. Equal loading of the gel was verified by Ponceau staining of the membrane after protein transfer. This experiment was repeated twice with the same results. (B) Relative EDS1 expression in 4-week-old cir1 and PR-1:LUC plants was determined using qPCR, with normalization to Actin2 expression levels. Each value is the mean of three independent biological repeats ± SEM. This experiment was repeated three times with the same results.