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. 2014 Oct 10;9(10):e109940. doi: 10.1371/journal.pone.0109940

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© 2014 Sands et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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We plated cells from pre-sort mixture and sorts on SC-leu- with 0.001% adenine, and photographed colonies after 48 hours. Left panel, pre sort mixture. Middle panel: sorted for long lifetime, enriched for white (BSY034, TFP) colonies. Right panel, sorted for shortened lifetime, enriched for red (BSY035, dCit-TFP) colonies. For each of three different trials we sorted three or four plates each of TFP and dCit-TFP strains. The plates here are representative examples.