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. 2014 Oct 10;9(10):e110404. doi: 10.1371/journal.pone.0110404

Table 1. Efficiency of simultaneous integration of GLVs.

Experiment Cells GLV1 GLV2 GLV3 # of colonies
#1 HAC/CHO 1C 2A 3A 8
#2 HAC/CHO 1C 2A 3A 7
#3 HAC/CHO 1C 2A 3A 6
#4 CHO 1C 2A 3A 0
#5 CHO 1C 2A 3A 0
#6 CHO 1C 2A 3A 1
#7 HAC/CHO - - - 0

3×105 target cells in a well of 12-well tissue culture plate were transfected with empty GLVs coding the indicated SIM cassettes together with expression vectors for Cre, Bxb1 integrase, and φC31 integrase. Cells were selected with HAT and resistant cells were stained with crystal violet to count the number of colonies.