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Outline of the gap-filling recombination repair assay.
A. A scheme of the sequential transformation protocol is presented. See text for details. B. A scheme of an assay that discriminates between strand-transfer and template-switching mechanisms of HR gap repair. If a T/G mismatch in the donor DNA is located at the position corresponding to the lesion, then gap filling by strand transfer will place a T opposite the lesion, whereas template switching will place a C at that position.
