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. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: J Mol Biol. 2008 Jun 18;381(4):803–809. doi: 10.1016/j.jmb.2008.06.031

Fig. 3.

Fig. 3

Hypothetical HR gap repair mechanisms involving two Holliday Junctions (HJ). HR is shown between a gap-lesion plasmid and a homologous donor containing a T:G mismatch at the site corresponding to the abasic site in the gap-lesion plasmid. (1), resolution of the two HJ in the vertical direction leads to gap filling via a strand transfer mechanism (product b), with a T opposite the site of the abasic site. (2), branch migration of the two HJ towards each other leads to dissolution (perhaps with the help of a topoisomerase), leading to a template switch gap recombination mechanism, which contains a C opposite the site of the abasic site. (3), resolution of the two HJ in the horizontal direction leads to template switching and strand transfer of the lesion-containing strand to the donor plasmid. This is also scored as template switching, because the selection is made for the plasmid that originally carried the gap-lesion. See text for further discussion.