Figure 1. Constitutive CN deficiency, but not IS treatment, confers anti-inflammatory properties to macrophages.

A Western blot confirming deletion of CnB and destabilization of CnA in macrophages from Cnb1^Δ/flox LysMCre⁺ mice (CN KO) compared with Cnb1^flox/flox LysMcre⁻ mice (Control).

B ELISA analysis of IL-10 protein in supernatants of CN-deleted and control macrophages (mean ± s.d.; n = 3).

C–E mRNA levels of Arg1 (C) and Mrc1 (D) and iNOS protein (E) in peritoneal macrophages from CN KO and control mice.

F, G In vivo imaging analysis of inflammation in CN KO and control mice after (F) sensitization with oxazolone in the right ear and (G) zymosan inoculation in the right hindpaw (n = 9 animals per genotype and condition).

H mRNA levels of IL-10, Arg1, Mrc1, and iNOS in peritoneal macrophages treated with CsA or FK506 (mean ± s.e.m.; n > 3).

Data information: *P < 0.05, **P < 0.01, ***P < 0.001.

Source data are available online for this figure.