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. 2014 Mar 28;33(9):1011–1026. doi: 10.1002/embj.201387009

Figure 1. Depletion of AFG3L2 causes mitochondrial defects.

Figure 1

  • Representative kymographs of axons (shown above respective kymographs) from neurons co-transfected with mito-mCherry and control or Afg3l2 siRNAs. Schematic of different color-coded transport types is shown below. Scale bars, 10 μm.
  • The percentage of mitochondria belonging to a given length bin was averaged from three independent experiments (120–150 mitochondria were measured in each experiment). Bars represent SEM. Chi-square test: P = 0.002.
  • Quantification of mitochondrial occupancy per axon (n = 3 experiments; 7–8 axons per experiment).
  • Quantification of mitochondrial transport types in the axon. Data represent mean ± SEM of three independent experiments. 7–8 axons from each experiment were analyzed. P-value was determined with Student's t-test.
  • Average mitochondrial velocity in the anterograde and retrograde direction. Data represent mean ± SEM of three independent experiments. The velocity of mitochondria from at least 5 axons per experiment was analyzed.