Figure 2.

Tissue specificity of Myh11 cre ER(T2) activity. 5-week old Myh11 creER(T2)^-/+ mTmG^-/+ mice were treated with tamoxifen (1 mg,IP) once a day for 5 days. 6 weeks later tissues were harvested and analyzed by confocal microscopy as described in ‘Methods’. A strong mEGFP signal can be seen only in smooth muscle cells of all the tissues examined including, bladder, colon, aorta, skeletal muscle and heart. No cre activity was detected in other cell types including skeletal muscle cells cardiac myocytes, endothelial cells or mucosal epithelial cells. SM-smooth muscle, LM-longitudinal muscle, CM-circular muscle, MP-myenteric plexus, MM-muscularis mucosa, EC-endothelial cells.