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. 2014 Oct 1;6:21. doi: 10.1186/2045-824X-6-21

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Copyright © 2014 Herring et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Previously differentiated VSMC contribute to early neointima formation. 5-week old male Myh11 creER(T2)^-/+ mTmG^-/+ mice were treated with tamoxifen (1 mg,IP) once a day for 5 days. Two weeks following the last tamoxifen injection the left carotid artery was ligated and tissues were harvested 14 days later. Expression of mTomato (red) and mEGFP (green) were visualized in sections obtained from 4 different mice. Nuclei were visualized by staining with DAPI (blue). All images are shown at the same magnification with the scale bar shown in the top left panel representing 50 μm.