Figure 3.

Contribution of oxidative stress in methylglyoxal-triggered endothelial NHE1 activation. (A) Means ± SEM (n = 6) of DCF-dependent fluorescence in EE2 ECs treated with MG (100 μM for 0–8 h) alone (MG) or co-treated with MG and cariporide (50 μM; 1 h prior to MG) or Tempol (300 μM; 1 h prior to MG). * indicates significant difference (p < 0.05) from MG alone. (B) Representative original Western blot and means ± SEM (n = 4) showing total NHE1 and phospho-NHE1 (14-3-3 binding motif at p-Ser⁷⁰³; relative to β-actin) determined in EE2 ECs treated with MG (100 μM for 1 h) alone or co-treated with MG and cariporide (50 μM; 1 h prior to MG) or Tempol (300 μM; 1 h prior to MG). * indicates significant difference (p < 0.05) from the absence of MG (Control). # indicates significant difference (p < 0.05) from MG alone.