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. Author manuscript; available in PMC: 2015 Oct 8.
Published in final edited form as: Cell Host Microbe. 2014 Oct 8;16(4):439–449. doi: 10.1016/j.chom.2014.09.003

Figure 1. T. cruzi sacrifices its flagellum during intracellular amastigogenesis.

Figure 1

(A–C) Fluorescent (top panels) and DIC overlay (bottom panels) showing T. cruzi flagellum in the extracellular trypomastigote stage (A) and separated from the newly formed amastigote (6–9 hrs post infection) in the host cell cytoplasm (B,C). The white and yellow arrows respectively indicate the basal body and DNA associated with the flagellum. (D) An intact flagellum is not evident in an infected host cell with single amastigote 9–12 hrs post-infection but flagellar origin PAR4 (HA-tagged) ‘particles’ appear in the host cell cytoplasm. Anti-PAR4 (a–c) or PAR4-HA used to track T. cruzi PAR4. 4',6-diamidino-2-phenylindole (DAPI) (A–C) or Propidium Iodide (PI) (D) stained DNA and anti-centrin marked the basal body (A–C). CN indicates host cell nucleus. Scale bar = 10µ. See also figures S1 and S2.