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. Author manuscript; available in PMC: 2015 Oct 8.
Published in final edited form as: Cell Host Microbe. 2014 Oct 8;16(4):439–449. doi: 10.1016/j.chom.2014.09.003

Figure 4. Over-expression of PAR4 in T. cruzi enhancements the generation of PAR4-specific CD8+ T cells.

Figure 4

(A) Relative expression levels of PAR4 in Tcwt and TcPAR4 trypomastigote stage (lysate) as determined by ELISA using serial dilutions of anti-PAR4 immune sera. (B) Intracellular IFN-γstaining of splenocytes derived from Tcwt or TcPAR4 infected (120 dpi) mice, restimulated with rPAR4(N), rOva or rOva-TSKb20 for 16hrs. Histograms are gated on CD8+ CD44+ lymphocytes, with the inset numbers indicating the percentage of IFN-γ producing CD8+ T cells. The bar graph summarizes data from an experiment with 3 mice/ group. (C) Cytolytic activity of splenocytes from TcPAR4 or Tcwt infected mice (96 dpi) against MC-PAR4(N) or MC-Ova target cells at different effector-target ratios. (D) Representative histograms comparing specific killing of CellTrace Violet (CTV) stained MC-PAR4(N) (CTVhi)/ MC-OVA (CTVlo) target cells inoculated into and recovered 18h later from the peritoneal cavity of naïve, Tcwt or TcPAR4 infected (300 dpi) mice. The inset numbers represent the relative proportions of recovered MC-PAR4(N) compared to MC-OVA. The bar graph shows the relative in vivo specific lysis of MC-PAR4(N) in Tcwt or TcPAR4 infected mice. Data represents an experiment with 3 mice/ group. All data representative of at least three similar experiments with the bar graphs presenting data as mean ± SEM. * or n.s indicate p≤ 0.05 or p>0.05 respectively, as determined by student t-test. See also figure S5