Skip to main content
. Author manuscript; available in PMC: 2015 Oct 8.
Published in final edited form as: Cell Host Microbe. 2014 Oct 8;16(4):439–449. doi: 10.1016/j.chom.2014.09.003

Figure 6. TcPAR4 immunization enhances resistance to a T. cruzi challenge.

Figure 6

(A) Schematic showing the methodology of infection (intra-perotoneal, i.p), cure, challenge (in the superficial subcutaneous tissue of each footpad) and assessment of protection in TcPAR4 or Tcwt infected mice. (B) Representative image from day 4 post-challenge showing the acute control of T. cruzi at the site of infection in naive (primi-challenge), and Tcwt- or TcPAR4-vaccinated mice. The parasite load in foot pad, represented by the fluorescent signal of T. cruzi expressing tdTomato (TctdTom) is determined by in vivo imaging. (C) The control of TctdTom challenge based on mean fluorescent signal of all feet in primi-challenged, Tcwt or TcPAR4 immunized mice at indicated time points. Data are from an experiment with 3 mice/group, which is representative of 3 separate trials. (D) T. cruzi DNA in skeletal muscle tissue of C57BL/6 mice immunized with Tcwt or TcPAR4 and challenged with TctdTom as determined by quantitative real-time PCR, 30 days post challenge. Horizontal bars represent the mean. Unimmunized (primi-infection) or naïve mice served as controls. The dotted line represents the threshold of detection for the assay. Data represented as mean ± SEM, from one of 2 separate experiments with 3–6 mice/ group. ** or * indicates p≤0.01 or ≤0.05 respectively by student t-test. See also figure S6.