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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Cell. 2014 Oct 9;159(2):388–401. doi: 10.1016/j.cell.2014.09.012

Figure 3. Heh2 specifically binds Snf7.

Figure 3

(A) Snf7 co-purifies with Heh2-GFP in vps4Δ cells. Western blots of proteins bound to Heh1- and Heh2-GFP from WT and vps4Δ cell extracts.

(B) Schematic of the topology and domain architecture of Heh2. LEM is Lap2-emerin-MAN1, MCHD is MAN1 C-terminal Homology Domain and TMD is transmembrane domain.

(C and D) Snf7 specifically interacts with the N-terminus of Heh2. GST-fusions of Heh2 domains were immobilized on GST-beads (bottom, coomassie panels) and incubated with buffer or whole cell extracts (WCE) as indicated in legend. HA fusions detected by Western blot.

See Figure S3.