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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Cell. 2014 Oct 9;159(2):388–401. doi: 10.1016/j.cell.2014.09.012

Figure 7. Vps4 destabilizes misassembled nups.

Figure 7

(A) An extra pool of Nup85 accumulates in the SINC of vps4Δ mothers. Plot of total Nup85-GFP fluorescence in mother (left) and daughter (right) nuclei measured after each of three divisions (normalized to first). Mean +/− s.d. from 3 experiments in which 5 cells (mother and daughter) were measured.

(B) Nup85-GFP is stabilized in vps4Δ cells when NPC assembly is inhibited. Micrographs of Nup85-GFP at either RT or at 34°C. Bars are 5µm.

(C) Nup85-GFP is degraded by the proteasome when NPC assembly is inhibited. Western blot of Nup85-GFP levels (with actin or CPY load controls) from experiments identical to that shown in B.

(D) Plot of the quantitation of Nup85-GFP levels in the indicated strains. Mean +/− s.d. from 3 experiments.

(E) NUP85 transcript levels decline in nic96-1vps4Δ cells. RT-qPCR analysis of NUP85 transcript compared to WT cells.

See Figure S5.