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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2014 Mar 31;2(7):655–667. doi: 10.1158/2326-6066.CIR-13-0209

Figure 1. Multiple myeloid cell subsets are present within tumors of different origin.

Figure 1

A, Analysis of myeloid cells subsets within subcutaneous 4T1 and Her2 tumors by flow cytometry. Hematopoietic cells were analyzed for the expression of CD11b and Gr1, and gated populations in the dot-plots were further analyzed for CD11c expression. B, MHCII expression by indicated subsets. C, Accumulation of CD11b+Gr1+ cells in the spleens of 4T1 tumor-bearing mice (top) and Gr1 expression (bottom). Numbers indicate percentage of cells for each gate or region. D, Dendrogram analysis based on hierarchical clustering of tumor-associated and steady-state myeloid cells (SI, small intestine, PC peritoneal cavity; Spl, spleen; BM, bone marrow; Tmr, tumor). Sample sizes are indicated in Materials and Methods. E, Hierarchical clustering of the same populations based on a list of 139 genes associated with DCs, macrophages or neutrophils.