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. 2014 May 13;33(13):1438–1453. doi: 10.15252/embj.201386907

Figure 6. Decline in NAD+ levels with age and reversal by nicotinamide mononucleotide (NMN).

Figure 6

  1. NAD+ measured from hearts excised from male 6-month- (n = 4), and 30-month-old (n = 4) mice.
  2. Relative NAD+ levels in hearts of young and aged mice treated with either PBS or NMN (n = 3 for each group).
  3. Relative NAD+ levels in testes from same mice as in (B).
  4. Lysates were prepared from testes of young and aged mice treated with either PBS or NMN and Western blotted for BubR1 and actin.
  5. Quantification of BubR1 protein levels from (D).
  6. Wild-type and Sirt2−/− MEFs treated with or without 1 mM NMN for 24 h and measured for relative NAD+ levels.
  7. Lysates from MEFs in (F) were Western blotted for BubR1, SIRT2, and tubulin.

Data information: Error bars represent SEM. P-values calculated using Student’s t-test (n = 3), *P < 0.05, **P < 0.005.