Figure 7. Assembly of F₁F_o-ATP synthase peripheral stalk subunits is impaired in ina22Δ and ina17Δ.

A [³⁵S]-labeled ATP synthase subunits were imported into isolated mitochondria from wild-type, ina22Δ (left panel), or ina17Δ (right panel) for indicated times and analyzed by SDS-PAGE and digital autoradiography.

B, C After import of radiolabeled F₁F_o-ATP synthase subunits into wild-type and ina22Δ (B) or ina17Δ (C) mitochondria, non-imported proteins were digested by proteinase K and samples analyzed by BN-PAGE followed by digital autoradiography.