Table 2.
Data collection and refinement statistics
| E. coli LeuRS:tRNALeu (UAA):IleAMS | |
|---|---|
| Data collection | |
| Space group | C2 |
| Cell dimensions | |
| a, b, c (Å) | 155.62, 67.58, 224.59 |
| α, β, γ (°) | 90.00, 105.0, 90.00 |
| Resolution (Å) | 46.5–2.5 (2.500–2.505) |
| Rsym | 17.0 (68.5) |
| I/σI | 7.0 (1.8) |
| Completeness (%) | 97.1 (98.7) |
| Redundancy | 3.17 (3.18) |
| Refinement | |
| Resolution (Å) | 2.5 |
| No. reflections work/free | 72414/3828 |
| Rwork/Rfree | 0.213/0.290 |
| No. atoms | |
| Protein | 6907[A]b/6897[D] |
| tRNA | 1756[B]/1692[E] |
| Ligand | 124 (4 × IleAMS) |
| Mg2+ | 1[B]/1[E] |
| Zn2+ | 1[A]/1[D] |
| Water/other | 306 |
| B-factors | |
| Protein | 22.5[A]/74.4[D] |
| tRNA | 30.13[B]/85.7[E] |
| Ligand | 9.6[A1]c/28.7[A2]c 49.7[D1]/144.5[D2] |
| Mg2+ | 25.2[B]/52.1[E] |
| Zn2+ | 65.7[A]/100.4[D] |
| Water | 19.8[Z] |
| R.M.S. deviations | |
| Bond lengths (Å) | 0.010 |
| Bond angles (°) | 1.502 |
a
Values in parentheses are for highest-resolution shell.
b
Values are for each molecule in the asymmetric unit with chain indicator given in square brackets.
c
Number 1 denotes Ile-AMS ligands bound into the synthetic site, and number 2 bound into the editing site.