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. 2014 Jun 5;33(15):1681–1697. doi: 10.15252/embj.201387549

Figure 5. Loss of vti1a results in a decreased number of releasable (primed) vesicles.

Figure 5

  1. Exocytosis induced by Ca2+-uncaging is reduced in vti1a nulls. Ca2+-uncaging at 0.5 s (at red arrow) led to a rapid increase in the global Ca2+-concentration (top) which resulted in vesicle fusion leading to an increase in cellular capacitance (middle), amperometric current (bottom, left ordinate), and cumulative charge (bottom, right ordinate).
  2. Quantification of the cellular capacitance prior to uncaging (cell size) and changes in capacitance at 1 s (burst) and 5 s (total) after uncaging. The sustained release (sust.) is the difference between total and burst secretion.
  3. Release kinetics is unaltered in vti1a nulls: capacitance curves from the middle panel of (A) scaled to their respective values at 1 s have similar shapes.

Data information: Bar diagram shows means ± SEM. Number of cells (n): wild-type: n = 34; vti1a null: n = 30. ***P < 0.001.

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