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. 2014 Jul 1;33(15):1713–1726. doi: 10.15252/embj.201387695

Figure 4. Analysis of VfAU1-LOV photoadduct lifetime, additional receptor tyrosine kinases and alternative LOV domains.

Figure 4

  1. MAPK/ERK pathway activation in response to blue light for HEK293 cells that were transfected with Opto-mFGFR1 or Opto-mFGFR1 with I472V substitution. This substitution reduces VfAU1-LOV photoadduct lifetime.
  2. MAPK/ERK pathway activation in response to blue light for HEK293 cells that were transfected with chimeric proteins of hEGFR1-ICD or hRET-ICD and VfAU1-LOV.
  3. MAPK/ERK pathway activation in response to blue light for HEK293 cells that were transfected with chimeric proteins of mFGFR1-ICD and NgPA1-LOV or OdPA1-LOV.

Data information: 24 h after transfection, cells were stimulated with blue light for 8 h followed by detection of luciferase. Light intensity was ˜2.5 μW/mm2. Mean values ± SEM for three to 16 independent experiments each performed in triplicates are shown.