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. 2014 Jul 1;33(15):1713–1726. doi: 10.15252/embj.201387695

Figure 6. Activation of cellular signalling with spatial precision.

Figure 6

  1. Spatially confined ERK1/2 phosphorylation in SPC212Opto-mFGFR1 cells. A illumination pattern of 16 equidistant circles was produced using a pinhole template. Stars mark circles in corners. Dashed line marks a single circle. Cells were stimulated with blue light for 5 min followed by fixation and staining. Scale bar: 2 mm.
  2. Spatially confined ERK1/2 phosphorylation in hBEOpto-mFGFR1 cells. A illumination pattern of two circles was produced using a pinhole template. Star marks the center of one circle, dashed line marks the other circle. Cells were stimulated with blue light for 5 min followed by fixation and staining. Scale bar: 5 mm.
  3. Spatially confined MAPK-dependent gene transcription in HEK293 cells. Thirty-four hours after transfection, cells stimulated with light for 8 h followed by live-cell detection of luciferase. Scale bar: 10 mm.

Data information: All images are unprocessed raw images. Light intensity was ˜2.5 μW/mm2.