Skip to main content
. 2014 May 16;33(12):1365–1382. doi: 10.15252/embj.201386874

Figure 7. ARRB1 regulates metabolism in prostate cancer cells.

Figure 7

  1. Principal Component Analysis of 1H NMR proton nuclear magnetic resonance measurements of cellular metabolites in control, wtARRB1 and nucARRB1 cells.
  2. 1H NMR proton nuclear magnetic resonance measurements of intracellular glucose and lactate, amino acids and TCA intermediate in GFP control and nucARRB1 cells.
  3. Kennedy pathway phospholipid intermediate metabolites choline, phosophocholine (PC), glycerophosphocholine (GPC) and total choline in GFP control and nucARRB1 cell lysates.
  4. Measurment of glucose flux to fumarate, malate and citrate using 1,2-13C-glucose and GC/MS in control and nucARRB1 cells.
  5. Glucose uptake and lactate secretion in GFP control and nucARRB1 cells.
  6. Growth plots of GFP control and nucARRB1 cells cultured in media supplemented with either glucose or galactose.
  7. Proliferation plots of LNCaP and C4-2 cells grown in media supplemented with either glucose or galactose.

Data information: For (B–E), values are mean ± s.e.m. of five replicates. For (F–G), N = 3, values are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.