Figure 5.
Fluorescence recovery rate in premyofibrils of both YFP-TPM1α (a) and YFP-TPM1κ (d) decreased in jasplakinolide medium as compared with control medium. In mature myofibrils, however, the recovery of both YFP-TPM1α (b) and YFP-TPM1κ (e) did not significantly differ in control and treated medium (b). In control medium, the recovery rates in premyofibrils of both YFP-TPM1α (a, upper curve) and YFP-TPM1κ (d, upper curve) were faster than in mature myotubes (upper curves, b and e) Graphs in (c) and (f) quantitate the slow and fast mobile fractions of YFP-TPM1α (c) and YFP-TPM1κ (f) in premyofibrils and mature myofibrils in medium with or without jasplakinolide.