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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Eur J Cancer. 2014 Sep 6;50(16):2866–2876. doi: 10.1016/j.ejca.2014.08.011

Figure 3. 4-OHT did not recruit ERα or SRC3 to the promoter of pS2 in MCF-7:PF cells.

Figure 3

(A) Recruitment of ERα at the pS2 proximal promoter. MCF-7:5C and MCF-PF cells were treated with vehicle (0.1% EtOH; con), E2 (10−9 mol/L), and 4-OHT (10−6 mol/L) for 45 min. ChIP assay was performed as in Materials and Methods. p<0.05, * compared with control, p<0.001, ** compared with control. (B) Recruitment of SRC3 at the pS2 proximal promoter. MCF-7:5C and MCF-PF cells were treated the same as above. ChIP assay was performed as described in Materials and Methods. p<0.05, * compared with control, p<0.001, ** compared with control. All the data shown are representative of at least three separate experiments with similar results.