Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Sep 25;9(1):272–284. doi: 10.1016/j.celrep.2014.08.047

Figure 3. Jak/Stat3 signaling mediates injury-dependent induction of the reprogramming gene, ascl1a.

Figure 3

(A) GFP immunofluorescence in 6-ascl1a:gfp fish shows that Jak inhibitors, P6 and JSI-124, applied at the time of retinal injury, inhibit injury-dependent transgene induction. (B) Diagram of ascl1a promoter constructs used to generate transgenic lines. (C) GFP immunofluorescence shows that a distal 1.5 kb fragment of the ascl1a promoter is required for injury-dependent transgene expression and that both consensus Stat3 sites located in this promoter fragment are necessary for this expression. BrdU+ cells indicate the injury site and a normal regenerative response. The asterisk marks the injury site (needle poke). Scale bar, 50 µm. INL, inner nuclear layer; dpi, days post injury. See also Figure S4.