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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Sep 25;9(1):272–284. doi: 10.1016/j.celrep.2014.08.047

Figure 5. Leptin signaling is necessary and sufficient for retina regeneration.

Figure 5

(A) RT-PCR analysis of mRNAs (from whole retina) encoding Leptin and Leptin receptor at various times after retinal injury. (B) In situ hybridization and BrdU immunofluorescence shows lepa, lepb and lepr RNAs are increased in BrdU+ MG-derived progenitors at the injury site. Asterisk marks the injury site (needle poke). (C, D) MO-mediated knockdown of the Leptin receptor inhibits the generation of BrdU+ MG-derived progenitors at 4 dpi; ***P<0.001, n=4. (E) qPCR shows Leptin receptor knockdown suppresses injury-dependent induction of reprogramming genes at 2 dpi; **P<0.01, n=4. (F) Intravitreal injection of recombinant human Leptin into the uninjured eye of 1016tuba1a:gfp fish stimulates GFP expression and BrdU incorporation in MG throughout the retina’s INL. Error bars, s.d. In (B, C,) arrows point to MG-derived progenitors. Scale bars, 20 µm (B, C); 50 µm (E). INL, inner nuclear layer. Primers are listed in Table S1. See also Figures S7.