Table 2. Gene expression analysis of laser-captured plaque CD68⁺ cells.

CD68+ cells from aortic plaques of Reversa mice were isolated using LCM. mRNA was isolated, amplified and analyzed by RT-PCR. mRNA levels are presented as relative levels normalized to HPRT.

		Normoglycemic		Diabetic

		Con α-miR	α-miR33	Con α-miR	α-miR33
α-miR33 targets	Abca1	116±21	96±11	61±7^#	110±12^*
α-miR33 targets	Abcg1	115±25	111±15	146±19	116±20

M2	Cd206	129±44	223±70	108±23	192±49
M2	Ym1	252±115	2479±1588^#	92±30	527±170^*

M1	Tnfα	116±27	87±14	236±75	99±22
	Il1β	138±40	90±21	304±74^#	63±12^*
	Nos2	159±63	121±54	179±54	43±15

Trafficking	Ccr2	122±26	153±60	349±77^#	126±16^*
	Ccr5	124±27	119±13	154±26	120±27
	Ccr7	137±33	189±80	139±50	131±41
	Netrin-1	123±38	188±46	195±45	155±41
	Unc5b	113±23	139±25	121±19	117±32

Adhesion	Vcam1	103±12	138±27	2537±51^#	110±16^*
Adhesion	Icam1	113±27	144±37	269±56	185±30

Data are expressed as mean ± SEM (n≥6 per group).

p≤0.05 vs. con α-miR normoglycemic;

p≤0.05 vs. con α-miR diabetic.

Table 2. Gene expression analysis of laser-captured plaque CD68+ cells.

Table 2. Gene expression analysis of laser-captured plaque CD68⁺ cells.