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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Thromb Haemost. 2014 Sep 30;12(10):1706–1716. doi: 10.1111/jth.12657

Figure 6. PAI-2 deficiency enhances neutrophil recruitment through elevated CXCL2 levels.

Figure 6

(A) Flow cytometry scatter plots and quantitation of flow cytometric results of cells released from Day 4 clots. Cells were double-stained with anti-CD11b and anti-Ly6B.2 as described in the methods. Cells were counted as neutrophils if they were Ly6B.2 positive or Ly6B.2 and CD11b double positive (upper 2 quadrants on scatter plots). Cells were counted as macrophages if they were only CD11b positive (lower right quadrant on scatter plots). The scatter plots show a typical results from one thrombi from each strain, and the bar graph represents the mean and SEM from 3-6 independent flow cytometry experiments. (Neutrophils: PAI-2 -/- vs WT p=0.006) (Macrophages: PAI-2 -/- vs WT p=0.04; PAI-1 -/- vs WT p<0.005). (B) CXCL2 and (C) CXCL1 ELISA of thrombus protein lysates on day 4 (n=3) (p=0.027).