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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Sep 25;9(1):118–128. doi: 10.1016/j.celrep.2014.08.042

Figure 2. 193-G2-001/Olaptesed secondary structure and binding kinetics.

Figure 2

(A) Pull-down binding assay of aptamer d-193-G2-001, using the biotinylated selection target d-SDF-1. Fitting with a 3 parameter algorithm revealed a Kd of 268 pM. (B) Secondary structure prediction for olaptesed, with potential base-pairs forming three hydrogen bonds depicted in red, and those forming two hydrogen bonds with each other shown in blue. (C) Biacore analysis of olaptesed pegol binding to immobilized human SDF-1α, at 37 °C under physiological buffer conditions. Raw data: black; fitted data: red.