Fig. 4. E2 repressed BSEP promoter transactivation in vitro through ERα.
(A) Huh 7 cells were transfected with phBSEP(−2.6kb), FXR and ERα or ERβ, followed by treatment with CDCA (10µM) or CDCA plus E2 (10nM). Luciferase activities were detected by the dual luciferase assays. (B) dose-response studies were performed with ERα and (C) E2. Data were presented as means ± SD of at least three individual experiments. One-way ANOVA was applied to analyze data in (A) and (C), followed by Tukey post-hoc test for multiple comparisons. The Student’s t-test was applied to pair-wise comparison in (B). * p<0.05 and ** p<0.01