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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Exp Neurol. 2014 Jun 21;0:65–75. doi: 10.1016/j.expneurol.2014.06.013

Figure 5. Inhibition of SCI-induced SOCS3 expression by shSOCS3.

Figure 5

A, NeuN+ neurons in the spinal cord were found to be infected by GFP-tagged lentivirus (arrow). Scale bar, 25 µm. B, Quantitative-PCR analysis of SOCS3 mRNA levels showed that shSOCS3 inhibited SCI-induced increases in endogenous SOCS3 expression 2 days after SCI in areas both rostral and caudal to the injured site. ***p < 0.001 compared to sham (con) group; ###p<0.001 compared to control lentivirus (pGipz)-treated group (ANOVA and Student-Newman-Keuls analyses). C–D, Western blot analysis showed SCI-induced increases in the expression of Bax and that the Bax/Bcl-2 ratio was reduced by shSOCS3 7 days after SCI (Tx) in areas both rostral (R) and caudal (C) to the injured site. ***p<0.001 compared to sham (con) group; ##p<0.01 compared to pGipz group (ANOVA and Student-Newman-Keuls analyses).