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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Exp Neurol. 2014 May 20;0:328–336. doi: 10.1016/j.expneurol.2014.05.014

Figure 1.

Figure 1

CFA induces CXCL1 mRNA and protein expression in the spinal cord. (A) Real-time PCR results show the increase of CXCL1 mRNA expression in the spinal cord at 6 h and 3 d after CFA injection. * P < 0.05, compared to naïve. # P < 0.05, compared to saline. n = 4 mice/group. (B) Elisa results show that CXCL1 protein expression was also increased in the spinal cord after CFA. ** P < 0.01, compared to naïve. ## P < 0.01, compared to saline. n = 5 mice/group. (C–F) Immunostaining shows the CXCL1 expression in the spinal cord in naïve (C) and CFA (D) animals. CFA increased CXCL1-IR in the spinal cord at 3 days after injection (D). (E–G) Double staining shows CXCL1 was colocalized with astrocytic marker, GFAP (E), but not with neuronal marker NeuN (F) or microglial marker CD11b (G).