Motor phenotype of EA2 mutant mice. Mice carrying various combinations of wild-type (+), knockin (EA2), and knockout (-) Cacna1a were tested for their acquisition of motor skills with the accelerating rotarod (A) and pole test (B) as well as grip strength as assessed by the cling test (C) and baseline locomotor activity (D). EA2/- mice (n=17), but not EA2/+ (n=11), +/−(n=10), or EA2/EA2 (n=11) mice showed reduced performance compared to +/+ (n=16) on the rotarod (***p<0.001, Tukey’s test) and pole test (*p<0.05, Tukey’s test). No significant differences between genotypes were found for cling test (F4,44=0.97, P>0.4) or locomotor activity (F4,27=0.27, P>0.5).